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KMID : 1036320130350020073
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Maxillofacial Plastic and Reconstructive Surgery
2013 Volume.35 No. 2 p.73 ~ p.81
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Smad4 Mediated TGF-β/BMP Signaling in Tooth Formation Using Smad4 Conditional Knockout Mouse
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Yoon Chi-Young
Baek Jin-A
Cho Eui-Sic
Ko Seung-O
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Abstract
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Purpose: Smad4 is a central mediator for transforming growth factor-${\beta}$ 수식 이미지/bone morphogenetic protein ($TGF-{\beta}/BMP$ 수식 이미지) signals, which are involved in regulating cranial neural crest cell formation, migration, proliferation, and fate determination. Accumulated evidences indicate that $TGF-{\beta}/BMP$ 수식 이미지 signaling plays key roles in the early tooth morphogenesis. However, their roles in the late tooth formation, such as cellular differentiation and matrix formation are not clearly understood. The objective of this study is to understand the roles of Smad4 in vivo during enamel and dentin formation through tissue-specific inactivation of Smad4.
Methods: We generated and analyzed mice with dental epithelium-specific inactivation of the Smad4 gene (K14-Cre:$Smad4^{fl/fl}$ 수식 이미지) and dental mesenchyme-specific inactivation of Smad4 gene (Osr2Ires-Cre:$Smad4^{fl/fl}$ 수식 이미지).
Results: In the tooth germs of K14-Cre:$Smad4^{fl/fl}$ 수식 이미지, ameloblast differentiation was not detectable in inner enamel epithelial cells, however, dentin-like structure was formed in dental mesenchymal cells. In the tooth germs of Osr2Ires-Cre:$Smad4^{fl/fl}$ 수식 이미지 mice, ameloblasts were normally differentiated from inner enamel epithelial cells. Interestingly, we found that bone-like structures, with cellular inclusion, were formed in the dentin region of Osr2Ires-Cre:$Smad4^{fl/fl}$ 수식 이미지 mice.
Conclusion: Taken together, our study demonstrates that Smad4 plays a crucial role in regulating ameloblast and odontoblast differentiation, as well as in regulating epithelial-mesenchymal interactions during tooth development.
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KEYWORD
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TGF-beta/BMP, SMAD4, Odontoblast, Ameloblast, Tooth development
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